Efficient CNS gene delivery by intravenous injection.
Nat Methods
; 7(11): 905-7, 2010 Nov.
Article
em En
| MEDLINE
| ID: mdl-20953176
ABSTRACT
We administered recombinant SV40-derived viral vectors (rSV40s) intravenously to mice with or without prior intraperitoneal injection of mannitol to deliver transgenes to the central nervous system (CNS). We detected transgene-expressing cells (mainly neurons) most prominently in the cortex and spinal cord; prior intraperitoneal mannitol injection increased CNS gene delivery tenfold. Intravenous injection of rSV40s, particularly with mannitol pretreatment, resulted in extensive expression of multiple transgenes throughout the CNS.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Medula Espinal
/
Córtex Cerebral
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Técnicas de Transferência de Genes
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Vírus 40 dos Símios
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Transgenes
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article