Genetically directing É-N, N-dimethyl-L-lysine in recombinant histones.
Chem Biol
; 17(10): 1072-6, 2010 Oct 29.
Article
em En
| MEDLINE
| ID: mdl-21035729
ABSTRACT
A molecular understanding of the biological phenomena orchestrated by lysine N(É)-methylation is impeded by the challenge of producing site-specifically and quantitatively methylated histones. Here, we report a general method that combines genetic code expansion and chemoselective reactions, for the quantitative, site-specific installation of dimethyl-lysine in recombinant histones. We demonstrate the utility of our method by preparing H3K9me2 and show that this modified histone is specifically recognized by heterochromatin protein 1 beta. Extensions of the strategy reported here will allow a range of chemoselective reactions (which have been used for residue-selective, but not site-selective protein modification) to be leveraged for site-specific protein modification.
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Base de dados:
MEDLINE
Assunto principal:
Histonas
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Lisina
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article