In silico QTL mapping of basal liver iron levels in inbred mouse strains.
Physiol Genomics
; 43(3): 136-47, 2011 Feb 11.
Article
em En
| MEDLINE
| ID: mdl-21062905
ABSTRACT
Both iron deficiency and iron excess are detrimental in many organisms, and previous studies in both mice and humans suggest that genetic variation may influence iron status in mammals. However, these genetic factors are not well defined. To address this issue, we measured basal liver iron levels in 18 inbred strains of mice of both sexes on a defined iron diet and found â¼4-fold variation in liver iron in males (lowest 153 µg/g, highest 661 µg/g) and â¼3-fold variation in females (lowest 222 µg/g, highest 658 µg/g). We carried out a genome-wide association mapping to identify haplotypes underlying differences in liver iron and three other related traits (copper and zinc liver levels, and plasma diferric transferrin levels) in a subset of 14 inbred strains for which genotype information was available. We identified two putative quantitative trait loci (QTL) that contain genes with a known role in iron metabolism Eif2ak1 and Igf2r. We also identified four putative QTL that reside in previously identified iron-related QTL and 22 novel putative QTL. The most promising putative QTL include a 0.22 Mb region on Chromosome 7 and a 0.32 Mb region on Chromosome 11 that both contain only one candidate gene, Adam12 and Gria1, respectively. Identified putative QTL are good candidates for further refinement and subsequent functional studies.
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Base de dados:
MEDLINE
Assunto principal:
Mapeamento Cromossômico
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Biologia Computacional
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Locos de Características Quantitativas
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Ferro
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Fígado
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Camundongos Endogâmicos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article