DNA adducts of ortho-toluidine in human bladder.
Biomarkers
; 16(2): 120-8, 2011 Mar.
Article
em En
| MEDLINE
| ID: mdl-21117897
ABSTRACT
BACKGROUND:
4-Aminobiphenyl (4-ABP) and o-toluidine are known human bladder carcinogens, but only 4-ABP-releasing DNA adducts are known.METHODS:
Determination of 4-ABP and o-toluidine-releasing DNA adducts in epithelial and submucosal bladder tissues of sudden death victims (SDV n=46), and bladder tumours (n=12) by gas chromatography/mass spectrometry.RESULTS:
Above background, 4 and 11 of 12 tumour samples contained adducts of 4-ABP (0.057 ± 0.125 fmol/µg DNA) and o-toluidine (8.72 ± 4.49 fmol/µg DNA), respectively. Lower adduct levels were present in both epithelial and submucosal bladder tissues of SDV (4-ABP 0.011 ± 0.022 and 0.019 ± 0.047 fmol/µg DNA; o-toluidine 0.24 ± 0.63 and 0.27 ± 0.70 fmol/µg DNA).CONCLUSION:
Detection of o-toluidine-releasing DNA adducts support the carcinogenicity of o-toluidine in the human bladder.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Toluidinas
/
Bexiga Urinária
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Neoplasias da Bexiga Urinária
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Adutos de DNA
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Células Epiteliais
Limite:
Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article