Effect of Rho signaling pathway on SiO2 induced α-SMA expression in human bronchial epithelial cells.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
; 35(11): 1145-9, 2010 Nov.
Article
em En
| MEDLINE
| ID: mdl-21131735
ABSTRACT
OBJECTIVE:
To investigate the role of Rho in SiO2 induced α-SMA expression in human bronchial epithelial cells (HBECs).METHODS:
HBECs were cultured and stimulated with SiO2. Immunocytochemistry and Western blot were used to detect the expression of α-SMA. The activity of Rho was determined by GST pull down assay. In the prevention experiment, SiO2-stimulated HBECs were incubated with Rho inhibitor Y27632, and the expression of α-SMA was examined by Western blot.RESULTS:
With SiO2 (0-300 µg/mL) treatment, the expression of α-SMA increased gradually, and 200 µg/mL of SiO2 led to the highest expression of α-SMA which was (5.09±1.98) times of the expression of α-SMA in the control group(P<0.01). HBECs treated with SiO2 (200 µg/mL) for indicated time (1, 2, 6, 12, and 24 h) showed an obvious increase of Rho activity(P<0.01). Y27632 inhibited SiO2-induced α-SMA expression significantly, and the inhibition rate of 20 and 30 µmol/L Y27632 was 68% and 75%, respectively (P<0.01).CONCLUSION:
Rho signaling pathway may mediate SiO2 induced α-SMA expression in HBECs.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Brônquios
/
Actinas
/
Dióxido de Silício
/
Células Epiteliais
/
Quinases Associadas a rho
Limite:
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article