Fluorescent in situ hybridization heating pretreatment: the key is temperature control.
Pathol Int
; 60(12): 792-4, 2010 Dec.
Article
em En
| MEDLINE
| ID: mdl-21140588
ABSTRACT
Fluorescent in situ hybridization (FISH) is a very useful tool for diagnostic and prognostic purposes in pathology. However, many laboratories still experience troubles when applying FISH to paraffin material. To overcome these difficulties, different pretreatments which include enzymatic digestion have been described. Usually, previous to digestion, a heating step is performed. The aim of this study was to compare the efficiency of the heating step with different buffers and different heating methods. We conclude that the main factor in the heating pretreatment is the temperature control, irrespective of the buffer used. Best results are obtained with any buffer by heating the slides to 99°C for 15 min followed by 10 min at room temperature.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Hibridização in Situ Fluorescente
/
Calefação
/
Linfoma
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article