Tight control of mitochondrial membrane potential by cytochrome c oxidase.
Mitochondrion
; 11(2): 334-41, 2011 Mar.
Article
em En
| MEDLINE
| ID: mdl-21147274
ABSTRACT
In the present work we have critically examined the use of the KCN-titration technique in the study of the control of the cellular respiration by cytochrome c oxidase (COX) in the presence of the mitochondrial membrane potential (Δψ(mito)) in HepG2 cells. We clearly show that the apparent high inhibition threshold of COX in the presence of maximal Δψ(mito) is due to the KCN-induced decrease of Δψ(mito) and not to a low control of COX on the mitochondrial respiration. The tight control exerted by COX on the Δψ(mito) provides further insights for understanding the pathogenetic mechanisms associated with mitochondrial defects in human neuromuscular degenerative disorders.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Complexo IV da Cadeia de Transporte de Elétrons
/
Potenciais da Membrana
/
Mitocôndrias
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article