Regulation of synaptic stability by AMPA receptor reverse signaling.
Proc Natl Acad Sci U S A
; 108(1): 367-72, 2011 Jan 04.
Article
em En
| MEDLINE
| ID: mdl-21173224
ABSTRACT
The establishment of neuronal circuits relies on the stabilization of functionally appropriate connections and the elimination of inappropriate ones. Here we report that postsynaptic AMPA receptors play a critical role in regulating the stability of glutamatergic synapses. Removal of surface AMPA receptors leads to a decrease in the number and stability of excitatory presynaptic inputs, whereas overexpression increases synapse number and stability. Furthermore, overexpression of AMPA receptors along with Neuroligin-1 in 293T cells is sufficient to stabilize presynaptic inputs from cortical neurons onto heterologous cells. The stabilization of presynaptic inputs by AMPA receptors is not dependent on receptor-mediated current and instead relies on structural interactions mediated by the N-terminal domain of the glutamate receptor 2 (GluR2) subunit. These observations indicate that transsynaptic signaling mediated by the extracellular domain of GluR2 regulates the stability of presynaptic terminals.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sinapses
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Transdução de Sinais
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Receptores de AMPA
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Espinhas Dendríticas
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article