[Screening and identification of differential expression genes related to stress fracture by cDNA microarray assay].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
; 28(11): 827-30, 2010 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-21241569
ABSTRACT
OBJECTIVE:
To evaluate the differentially expressed genes between the Stress fracture (SF) cases and controls.METHODS:
Total RNA was extracted and purified from peripheral blood sample of 3 SF cases and 3 controls who conducted a 11 matched case-control study, then used for Human Genome Array analysis. The hybridization data were analyzed using SAM software. Parts of these genes were analyzed and identified by real-time PCR.RESULTS:
Upregulated and downregulated genes were 22 and 1, respectively. Thus the highest ratio and most significant cytokine was tumor necrosis factor receptor superfamily, member 10c (TNFRSF10C). The result of real-time PCR shows that TNFRSF10C was over-expressed in 3 cases and low-expressed in 1 case.CONCLUSION:
Obvious difference exists in gene expression between SF cases and controls, showing there may be a lot of genes involving in the occurrence and development of SF. Meanwhile, the identification of the specific genes is helpful for biomechanics study, early diagnosis and screening of SF.
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Base de dados:
MEDLINE
Assunto principal:
Fraturas de Estresse
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
/
Receptores Chamariz do Fator de Necrose Tumoral
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
/
Screening_studies
Limite:
Adult
/
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2010
Tipo de documento:
Article