Fast liquid chromatography combined with mass spectrometry for the analysis of metabolites and proteins in human body fluids.
Anal Bioanal Chem
; 399(8): 2635-44, 2011 Mar.
Article
em En
| MEDLINE
| ID: mdl-21253711
ABSTRACT
In the last decade various analytical strategies have been established to enhance separation speed and efficiency in high performance liquid chromatography applications. Chromatographic supports based on monolithic material, small porous particles, and porous layer beads have been developed and commercialized to improve throughput and separation efficiency. This paper provides an overview of current developments in fast chromatography combined with mass spectrometry for the analysis of metabolites and proteins in clinical applications. Advances and limitations of fast chromatography for the combination with mass spectrometry are discussed. Practical aspects of, recent developments in, and the present status of high-throughput analysis of human body fluids for therapeutic drug monitoring, toxicology, clinical metabolomics, and proteomics are presented.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Líquidos Corporais
/
Proteínas
/
Cromatografia Líquida de Alta Pressão
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article