Engineering aspartate transcarbamylase.
Biochimie
; 72(8): 609-16, 1990 Aug.
Article
em En
| MEDLINE
| ID: mdl-2126465
ABSTRACT
Aspartate transcarbamylase from Escherichia coli is one of the most extensively studied regulatory enzymes as a model of cooperativity and allostery. Numerous methods are used to engineer variants of this molecule random and site-directed mutagenesis, dissociation and reassociation of the catalytic and regulatory subunits and chains, construction of hybrids made from normal and modified subunits or chains, interspecific hybrids and construction of chimeric enzymes. These methods provide detailed information on the regions, domains, interfaces and aminoacid residues which are involved in the mechanism of co-operativity between the catalytic sites, and of regulation by the antagonistic effectors CTP and ATP. These effectors induce the transmission of intramolecular signals whose pathways begin to be delineated.
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Base de dados:
MEDLINE
Assunto principal:
Aspartato Carbamoiltransferase
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article