[Effect of Shuyusan on neuropeptide Y and serotonin expression in the hippocampal neurons of rats with chronic mild unpredictable stress-induced depression].
Nan Fang Yi Ke Da Xue Xue Bao
; 31(1): 113-6, 2011 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-21269971
ABSTRACT
OBJECTIVE:
To investigate the effect of Shuyusan decoction on neuropeptide Y (NPY) and serotonin (5-HT) expression in the hippocampus and plasma of rats with chronic mild unpredictable stressors depression.METHODS:
Fifty Wistar rats were randomly divided into 5 groups, namely the normal control group, model group, fluoxetine group, and high- and low-dose Shuyusan groups. Except for those in the normal control group, all the rats were subjected to chronic mild unpredicted stress for 21 consecutive days with corresponding treatments. Open-field test was used to assess the behavioral changes of the rats. The content of NPY in the hippocampus and plasma was detected by competitive enzyme-linked immunosorbent assay, and immunocytochemistry was used to determine the expression of 5-HT in the hippocampus.RESULTS:
NPY levels in the hippocampus and plasma was significantly decreased in the model group as compared with that in the normal control group (P<0.05). Treatments with fluoxetine and high-dose Shuyusan both significantly increased NPY levels in the hippocampus and plasma in the depressive rats (P<0.05), resulting also in significantly increased 5-HT-immunoreactive neurons in the cerebral cortex and hippocampus and the average optical density (P<0.05).CONCLUSION:
Shuyusan decoction can effectively increase plasma and hippocampus NPY levels and the number of 5-HT-positive neurons in the cerebral cortex and happocampus of rats with chronic mild unpredictable stress-induced depression.
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Base de dados:
MEDLINE
Assunto principal:
Neuropeptídeo Y
/
Medicamentos de Ervas Chinesas
/
Serotonina
/
Depressão
/
Hipocampo
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2011
Tipo de documento:
Article