Characterization of a novel human mast cell line that responds to stem cell factor and expresses functional FcεRI.
J Allergy Clin Immunol
; 127(3): 815-22.e1-5, 2011 Mar.
Article
em En
| MEDLINE
| ID: mdl-21281958
ABSTRACT
BACKGROUND:
Studies of human mast cells (MCs) are constrained by the paucity of functional cell lines, the expense of maintaining MCs in culture, and technical complexities.OBJECTIVE:
We derived and characterized a human MC line that arose spontaneously from a culture of nontransformed hematopoietic progenitor cells.METHODS:
CD34(+) enriched mononuclear cells derived from a donor with aspirin-exacerbated respiratory disease were cultured for 8 weeks with stem cell factor and IL-6 and with IL-3 for the first week only. The cells (termed LUVA cells) survived and proliferated without further addition of any growth factors and have been maintained in culture for approximately 2 years.RESULTS:
LUVA cells possess metachromatic cytoplasmic granules that are immunoreactive for tryptase, cathepsin G, and carboxypeptidase A3. They express transcripts encoding FcεRI, c-kit, chymase, tryptase, histidine decarboxylase, carboxypeptidase A3, and the type 1 receptor for cysteinyl leukotrienes. Flow cytometry confirmed uniform expression of FcεRI, c-kit, and FcγRII. FcεRI cross-linkage induced the release of ß-hexosaminidase, prostaglandin D(2), thromboxane A(2), and macrophage inflammatory protein 1ß. Immortalization was not associated with either a known genomic mutation of c-kit in the donor or a somatic mutation of c-kit within the cells, and it was not associated with c-kit autophosphorylation.CONCLUSIONS:
LUVA cells are an immortalized human MC line that can be maintained without stem cell factor and display high levels of normally signaling c-kit and FcεRI. These cells will prove valuable for functional human MC studies.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Receptores de IgE
/
Fator de Células-Tronco
/
Mastócitos
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article