DNA phosphorothioation is widespread and quantized in bacterial genomes.
Proc Natl Acad Sci U S A
; 108(7): 2963-8, 2011 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-21285367
ABSTRACT
Phosphorothioate (PT) modification of DNA, with sulfur replacing a nonbridging phosphate oxygen, was recently discovered as a product of the dnd genes found in bacteria and archaea. Given our limited understanding of the biological function of PT modifications, including sequence context, genomic frequencies, and relationships to the diversity of dnd gene clusters, we undertook a quantitative study of PT modifications in prokaryotic genomes using a liquid chromatography-coupled tandem quadrupole mass spectrometry approach. The results revealed a diversity of unique PT sequence contexts and three discrete genomic frequencies in a wide range of bacteria. Metagenomic analyses of PT modifications revealed unique ecological distributions, and a phylogenetic comparison of dnd genes and PT sequence contexts strongly supports the horizontal transfer of dnd genes. These results are consistent with the involvement of PT modifications in a type of restriction-modification system with wide distribution in prokaryotes.
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
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Vibrionaceae
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DNA Bacteriano
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Genoma Bacteriano
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Transferência Genética Horizontal
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Oligonucleotídeos Fosforotioatos
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Genes Bacterianos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article