The typing of Trypanosoma evansi isolates using mobile genetic element (MGE) PCR.
Parasitol Res
; 108(6): 1583-7, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21287202
ABSTRACT
The mobile genetic element PCR (MGE-PCR) is a simple and sensitive technique that can be used to detect genetic variability in Trypanosoma brucei ssp. To investigate the reliability of MGE-PCR in genotyping Trypanosoma evansi, stocks that were isolated directly from camels and after their respective passage in mice were analyzed. Construction of a dendrogram using the MGE-PCR banding profiles revealed a clear distinction between T. evansi and T. brucei, as well as discriminating the T. evansi strains (T. evansi with minicircle types B and A). A minor host-dependent clustering shows a genetic difference of <15%. Changes in the banding profiles were observed after serial passage of T. evansi type B in mice, while those of T. evansi type A were identical. It is apparent that significant random insertion mobile element positional variation occurs when T. evansi isolates are introduced into a new host, a factor that needs to be considered when MGE-PCR is used to determine genetic variation in T. evansi isolates that have different host origins.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Trypanosoma
/
Reação em Cadeia da Polimerase
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Sequências Repetitivas Dispersas
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article