Comment on "Unbiased statistical analysis for multi-stage proteomic search strategies".
J Proteome Res
; 10(4): 2123-7, 2011 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-21288048
ABSTRACT
Everett et al. recently reported on a statistical bias that arises in the target-decoy approach to false discovery rate estimation in two-pass proteomics search strategies as exemplified by X!Tandem. This bias can cause serious underestimation of the false discovery rate. We argue here that the "unbiased" solution proposed by Everett et al., however, is also biased and under certain circumstances can also result in a serious underestimate of the FDR, especially at the protein level.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Software
/
Bases de Dados de Proteínas
/
Proteômica
/
Ferramenta de Busca
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article