Drosophila katanin is a microtubule depolymerase that regulates cortical-microtubule plus-end interactions and cell migration.
Nat Cell Biol
; 13(4): 361-70, 2011 Apr.
Article
em En
| MEDLINE
| ID: mdl-21378981
ABSTRACT
Regulation of microtubule dynamics at the cell cortex is important for cell motility, morphogenesis and division. Here we show that the Drosophila katanin Dm-Kat60 functions to generate a dynamic cortical-microtubule interface in interphase cells. Dm-Kat60 concentrates at the cell cortex of S2 Drosophila cells during interphase, where it suppresses the polymerization of microtubule plus-ends, thereby preventing the formation of aberrantly dense cortical arrays. Dm-Kat60 also localizes at the leading edge of migratory D17 Drosophila cells and negatively regulates multiple parameters of their motility. Finally, in vitro, Dm-Kat60 severs and depolymerizes microtubules from their ends. On the basis of these data, we propose that Dm-Kat60 removes tubulin from microtubule lattice or microtubule ends that contact specific cortical sites to prevent stable and/or lateral attachments. The asymmetric distribution of such an activity could help generate regional variations in microtubule behaviours involved in cell migration.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Movimento Celular
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Adenosina Trifosfatases
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Proteínas de Drosophila
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Drosophila melanogaster
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Microtúbulos
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article