Identification of a single amino acid required for APOBEC3 antiretroviral cytidine deaminase activity.
J Virol
; 85(11): 5691-5, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21430060
ABSTRACT
During studies of APOBEC3 (A3) anti-human immunodeficiency virus type 1 (anti-HIV-1) mechanisms, we identified a single cysteine at position 320 (C320) that disrupts A3DE activity. This residue is located in the recently identified DNA binding domain in A3G. Replacing C320 with a corresponding tyrosine from A3F (Y307) increased A3DE antiviral activity more than 20-fold. Conversely, replacing A3F Y307 with a cysteine or inserting a similar cysteine into A3B or A3G disrupted the anti-HIV activity of A3. Further investigation uncovered that C320 significantly reduces A3DE catalytic activity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
HIV-1
/
Cisteína
/
Citidina Desaminase
/
Citosina Desaminase
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article