Ligand-affinity cloning and structure of a cell surface heparan sulfate proteoglycan that binds basic fibroblast growth factor.
Proc Natl Acad Sci U S A
; 87(18): 6985-9, 1990 Sep.
Article
em En
| MEDLINE
| ID: mdl-2144898
ABSTRACT
Expression cloning of cDNAs encoding a basic fibroblast growth factor (FGF) binding protein confirms previous hypotheses that this molecule is a cell-surface heparan sulfate proteoglycan. A cDNA library constructed from a hamster kidney cell line rich in FGF receptor activity was transfected into a human lymphoblastoid cell line. Clones expressing functional basic FGF binding proteins at their surfaces were enriched by panning on plastic dishes coated with human basic FGF. The amino acid sequence deduced from the isolated cDNAs revealed several interesting features, including hydrophobic signal and transmembrane domains that flank an extracellular region containing six potential attachment sites for glycosaminoglycan side chains. The structure also contains a short hydrophilic cytoplasmic tail sequence homologous to previously reported actin binding domains. Binding of basic FGF to cells expressing the binding protein could be inhibited by heparin and heparan sulfate but not by chondroitin sulfate, dermatan sulfate, or keratan sulfate. In addition to binding basic FGF, this protein or related surface proteins may function as an initial cellular attachment site for other growth factors and for viruses, such as herpes simplex virus.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteoglicanas de Sulfatos de Condroitina
/
Proteoglicanas
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Fatores de Crescimento de Fibroblastos
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Glicosaminoglicanos
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Heparitina Sulfato
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article