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Nestin expression is lost in ventricular fibroblasts during postnatal development of the rat heart and re-expressed in scar myofibroblasts.
Béguin, Pauline C; Gosselin, Hugues; Mamarbachi, Maya; Calderone, Angelino.
Afiliação
  • Béguin PC; Research Center, Montreal Heart Institute, Montreal, Quebec, Canada.
J Cell Physiol ; 227(2): 813-20, 2012 Feb.
Article em En | MEDLINE | ID: mdl-21503881
ABSTRACT
Studies have reported that the intermediate filament protein nestin was expressed in various non-stem/progenitor cells during development, downregulated during postnatal growth and re-expressed following injury. The present study tested the hypothesis that an analogous paradigm was prevalent for ventricular fibroblasts. In the neonatal rat heart, nestin protein levels were significantly higher than the adult heart and the isolation of cardiac cells revealed a selective expression in ventricular fibroblasts. In adult ventricular fibroblasts, nestin protein expression was markedly lower compared to neonatal ventricular fibroblasts. Following ischemic damage to the rat heart, nestin staining was detected in a subpopulation of scar myofibroblasts (37%) and the percentage of immunoreactive cells was greater than adult ventricular fibroblasts (7%) but significantly lower than neonatal ventricular fibroblasts (86%). Moreover, dissimilar rates of (3)H-thymidine uptake were observed among the fibroblast populations and may be related in part to the disparate percentage of nestin(+) cells. To assess the role of nestin in DNA synthesis, neonatal ventricular fibroblasts were infected with a lentivirus containing a shRNAmir directed against the intermediate filament protein. The partial depletion of nestin expression in neonatal ventricular fibroblasts significantly reduced basal DNA synthesis, in the absence of an apoptotic response. Thus, postnatal development of the rat heart was associated with a selective loss of nestin expression in ventricular fibroblasts and subsequent induction in a subpopulation of myofibroblasts following ischemic injury. The re-expression of nestin in scar myofibroblasts may represent an adaptive response to enhance their proliferative rate and accelerate the healing process.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cicatriz / Miofibroblastos / Fibroblastos / Ventrículos do Coração / Proteínas de Filamentos Intermediários / Miocárdio / Proteínas do Tecido Nervoso Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cicatriz / Miofibroblastos / Fibroblastos / Ventrículos do Coração / Proteínas de Filamentos Intermediários / Miocárdio / Proteínas do Tecido Nervoso Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2012 Tipo de documento: Article