Purification, crystallization and preliminary X-ray diffraction analysis of ThiM from Staphylococcus aureus.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 67(Pt 4): 479-81, 2011 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-21505246
ABSTRACT
ThiM [5-(hydroxyethyl)-4-methylthiazole kinase; EC 2.7.1.50] from Staphylococcus aureus is an essential enzyme of thiamine or vitamin B(1) metabolism and has been crystallized by the vapour-diffusion method. The crystals belonged to the primitive space group P1, with unit-cell parameters a = 62.06, b = 62.40, c = 107.82â
Å, α = 92.25, ß = 91.37, γ = 101.48° and six protomers in the unit cell, corresponding to a packing parameter V(M) of 2.3â
Å(3)â
Da(-1). Diffraction data were collected to 2.1â
Å resolution using synchrotron radiation. The phase problem was solved by molecular replacement.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Staphylococcus aureus
/
Fosfotransferases (Aceptor do Grupo Álcool)
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article