Subcellular features revealed on unfixed rat brain sections by phase imaging.
Analyst
; 136(11): 2270-6, 2011 Jun 07.
Article
em En
| MEDLINE
| ID: mdl-21509366
For sectioned biologic tissues, atomic force microscopy (AFM) topographic images alone hardly provide adequate information leading to revealing biological structures. We demonstrate that phase imaging in amplitude-modulation AFM is a powerful tool in mapping structures present on the surface of unfixed rat brains sections. The contrast in phase images is originated from the difference in mechanical properties between biological structures. Visualization of the native state of biological structures by way of their mechanical properties provides a complementary technique to more traditional imaging techniques such as optical and electron microscopy.
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Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Microscopia de Força Atômica
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article