Evaluation of next-generation sequencing software in mapping and assembly.
J Hum Genet
; 56(6): 406-14, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21525877
ABSTRACT
Next-generation high-throughput DNA sequencing technologies have advanced progressively in sequence-based genomic research and novel biological applications with the promise of sequencing DNA at unprecedented speed. These new non-Sanger-based technologies feature several advantages when compared with traditional sequencing methods in terms of higher sequencing speed, lower per run cost and higher accuracy. However, reads from next-generation sequencing (NGS) platforms, such as 454/Roche, ABI/SOLiD and Illumina/Solexa, are usually short, thereby restricting the applications of NGS platforms in genome assembly and annotation. We presented an overview of the challenges that these novel technologies meet and particularly illustrated various bioinformatics attempts on mapping and assembly for problem solving. We then compared the performance of several programs in these two fields, and further provided advices on selecting suitable tools for specific biological applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Software
/
Mapeamento Cromossômico
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Biologia Computacional
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Sequenciamento de Nucleotídeos em Larga Escala
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article