Specific detection of antibodies to different flaviviruses using a new immune complex ELISA.
Med Microbiol Immunol
; 200(4): 233-9, 2011 Nov.
Article
em En
| MEDLINE
| ID: mdl-21533786
ABSTRACT
Immune complex (IC) ELISAs for IgG antibodies to various virus antigens have turned out to be both highly specific and sensitive. During incubation of a labelled antigen with the serum samples, ICs are formed, which bind to microtiter plates coated with rheumatoid factor (RF) IgM. Here, we describe an improved coating of the solid-phase support comparing various Fc-receptor molecules. IC ELISAs were applied to detect human IgG antibodies to the highly virus-specific ED3 domain of West Nile- and tick-borne encephalitis virus envelopes. Compared with other Fc-receptor molecules like RF or C1q, FcγRIIA (CD32) turned out to bind the ICs composed of IgG antibodies and peroxidase-labelled ED3 antigens more efficiently. Due to low background reactions, sera could be tested at a dilution of 110. Moreover, using CD32 instead of RF coating, anti-flavivirus antibodies could be detected in various animal species.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Febre do Nilo Ocidental
/
Ensaio de Imunoadsorção Enzimática
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Encefalite Transmitida por Carrapatos
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Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article