Characterizing the proteome of the human dorsolateral prefrontal cortex by shotgun mass spectrometry.
Proteomics
; 11(11): 2347-53, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21538886
ABSTRACT
The studies of neuropsychiatric disorders would be facilitated by enhanced knowledge of the dorsolateral prefrontal cortex (DLPFC) proteome. To construct a data set of human DLPFC proteins, protein extracts were prepared from 12 postmortem brains focussing on the DLPFC region (Brodmann area 9) and analyzed using a combined gel electrophoresis and shotgun mass spectrometry approach, featuring data-independent label-free nanoflow liquid chromatography mass spectrometry (nLC-MS(E)). The detected mass/time features were aligned and annotated using the results from ProteinLynx Global Server. The resulting data set comprised 488 unique and accurately identified proteins, with stringent identification by a minimum of two distinct peptides detected at least in >75% of samples. These proteins were involved predominantly in cytoskeletal architecture, metabolism, transcription/translation, and synaptic function. Combination of this data set with that obtained by our previous characterization of the same brain region results in a total of 755 unique proteins, making this the most comprehensive analysis of this important brain region to date.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
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Mapeamento de Peptídeos
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Córtex Pré-Frontal
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Proteoma
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Proteômica
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Proteínas do Tecido Nervoso
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article