Cloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA intein.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 67(Pt 5): 623-6, 2011 May 01.
Article
em En
| MEDLINE
| ID: mdl-21543876
ABSTRACT
The RadA intein from the hyperthermophilic archaebacterium Pyrococcus horikoshii was cloned, expressed and purified for subsequent structure determination. The protein crystallized rapidly in several conditions. The best crystals, which diffracted to 1.75 Å resolution, were harvested from drops consisting of 0.1 M HEPES pH 7.5, 3.0 M NaCl and were cryoprotected with Paratone-N before flash-cooling. The collected data were processed in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 58.1, b = 67.4, c = 82.9 Å. Molecular replacement with Rosetta using energy- and density-guided structure optimization provided the initial solution, which is currently under refinement.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Arqueais
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Pyrococcus horikoshii
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Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article