Peptide secondary structure modulates single-walled carbon nanotube fluorescence as a chaperone sensor for nitroaromatics.
Proc Natl Acad Sci U S A
; 108(21): 8544-9, 2011 May 24.
Article
em En
| MEDLINE
| ID: mdl-21555544
ABSTRACT
A class of peptides from the bombolitin family, not previously identified for nitroaromatic recognition, allows near-infrared fluorescent single-walled carbon nanotubes to transduce specific changes in their conformation. In response to the binding of specific nitroaromatic species, such peptide-nanotube complexes form a virtual "chaperone sensor," which reports modulation of the peptide secondary structure via changes in single-walled carbon nanotubes, near-infrared photoluminescence. A split-channel microscope constructed to image quantized spectral wavelength shifts in real time, in response to nitroaromatic adsorption, results in the first single-nanotube imaging of solvatochromic events. The described indirect detection mechanism, as well as an additional exciton quenching-based optical nitroaromatic detection method, illustrate that functionalization of the carbon nanotube surface can result in completely unique sites for recognition, resolvable at the single-molecule level.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Chaperonas Moleculares
/
Nanotubos de Carbono
/
Hidrocarbonetos Aromáticos
/
Nitrocompostos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article