Size and specificity of radiopharmaceuticals for sentinel lymph node detection.
Acta Radiol
; 52(7): 774-8, 2011 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-21558148
ABSTRACT
BACKGROUND:
Biological performance of radiotracers for sentinel node detection analyzed in the light of molecular design and dimension is not widely available.PURPOSE:
To evaluate the effect of dextran molecular size and the presence of tissue-binding units (mannose) within the model of (99m)Tc-carbonyl conjugate for sentinel lymph node detection. MATERIAL ANDMETHODS:
Four dextran conjugates with and without mannose in the chemical backbone were included. All polymers were radiolabeled using the precursor [(99m)Tc(OH(2))(3)(CO)(3)](+). Radiolabeling conditions targeted the best radiochemical purity and specific activity for each radiopharmaceutical, and partition coefficients were also defined. Lymphoscintigraphy and ex-vivo biodistribution in popliteal lymph node, liver and kidneys were performed in Wistar rats. The effects of molecular weight and mannose presence were assessed by a two-level factorial design.RESULTS:
Radiochemical purity was indirectly related to molecular weight and presence of mannose in the polymer structure. All products were able to detect popliteal lymph node, however, uptake was strongly influenced by use of mannose (4-fold higher). Excretion was similarly modulated by differences in molecular weight. Mannose-enhanced lymph node uptake and higher molecule size in the range under study benefitted lymphoscintigraphic performance.CONCLUSION:
Screening of radiopharmaceuticals for lymphoscintigraphy might improve with attention to the mentioned physico-chemical features of the molecule.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Compostos Radiofarmacêuticos
/
Biópsia de Linfonodo Sentinela
/
Metástase Linfática
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article