Neuroprotective fractalkine in fetal alcohol syndrome.
Am J Obstet Gynecol
; 204(5): 400.e1-3, 2011 May.
Article
em En
| MEDLINE
| ID: mdl-21572545
ABSTRACT
OBJECTIVE:
Neuroprotective peptides (NAP+SAL) can prevent some alcohol-induced damage in fetal alcohol syndrome(FAS). Fractalkine, a chemokine constitutively expressed in the CNS reduces neuronal death from activated microglia. Using a model of FAS we evaluated if fractalkine is altered and if NAP+SAL work through fractalkine. STUDYDESIGN:
Using a FAS model, C57BL6/J-mice were treated on gestational day 8 with alcohol (0.03 mL/g), placebo or alcohol+peptides. Embryos were harvested after 6h(E8) and 10 days later(E18). Fractalkine was measured in the protein lysate (Luminex xMAP). Statistical analysis included Kruskal-Wallis.RESULTS:
Fractalkine was significantly elevated at 6h (median 341 pg/ml, range 263-424 pg/ml) vs. controls (median 228 pg/ml, range 146-332 pg/ml; P<.001). NAP+SAL prevented the alcohol-induced increase (median 137, range 97-255 pg/ml, P<.001). At E18, fractalkine levels were similar in all groups (P=0.7).CONCLUSION:
Prenatal alcohol exposure acutely elevates fractalkine, perhaps in an effort to counter the alcohol toxicity. Pre-treatment with NAP+SAL prevents the acute increase in fractalkine.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Etanol
/
Quimiocina CX3CL1
/
Transtornos do Espectro Alcoólico Fetal
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article