[Instantaneous expression aFGF-GFP fusion gene in safflower].

ABSTRACT

OBJECTIVE: To study the instantaneous expression aFGF-GFP fusion gene in Carthamus tinctorius. METHOD: Molecular biology methods were applied to construct aFGF and GFP fusion gene vector, it is transformed into C. tinctorius by Agrobacterium tumefaciens, forming the resistant callus, fluorescence microscopy was used for detection. RESULT: aFGF gene and GFP gene were amplified by PCR reaction. It was successfully constructed plant fluorescence expression vector pCAMBIA139035SaFGF-GFP, it was used to transform C. tinctorius, and the acquired resistance calli showed strong green fluorescence under UV light. CONCLUSION: The expression of GFP in resistance C. tinrictorius calli is good, it is indicated that aFGF gene in plant cells has also been expressed.