A rapid and economical method of preparing radioiodinated cyclic nucleotide derivatives for use in radioimmunoassays.
J Immunoassay
; 11(1): 109-18, 1990.
Article
em En
| MEDLINE
| ID: mdl-2159027
ABSTRACT
2'-O-succinyladenosine 3'5'-cyclic monophosphate tyrosyl methyl ester (ScAMP-TME) and 2'-O-succinylguanosine 3'5'-cyclic monophosphate tyrosyl methyl ester (ScGMP-TME) were radioiodinated using chloramine T and Na125I. The resulting radiolabeled cyclic nucleotide derivatives, ScAMP-125I-TME and ScGMP-125I-TME, were subsequently purified by reverse-phase chromatography on Sep-Pak C18 cartridges (Waters Associates, Milford, MA) and tested as tracers in sensitive radioimmunoassays for cAMP and cGMP, respectively. Purified ScAMP-125I-TME and ScGMP-125I-TME functioned in the respective radioimmunoassays for up to 12 weeks when suspended in a 11 (vv) mixture of n-propanol and 20 mM sodium acetate, pH 6.0. Thus, this purification method enables rapid and economical preparation of tracers for cyclic nucleotide radioimmunoassays. Furthermore, our findings suggest that reverse-phase chromatography may be applicable to the purification of other small polar molecules to which tyrosyl groups have been added for the purpose of radioiodination.
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Base de dados:
MEDLINE
Assunto principal:
Tirosina
/
AMP Cíclico
/
GMP Cíclico
Tipo de estudo:
Health_economic_evaluation
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article