Direct comparison of four adeno-associated virus serotypes in mediating the production of antiangiogenic proteins in mouse muscle.
Cancer Invest
; 29(5): 353-9, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21599511
ABSTRACT
To determine the adeno-associated virus (AAV) serotype that most efficiently mediates muscle expression of antiangiogenic proteins, we injected four different serotype (1, 2, 7, and 8) AAV vectors encoding mouse endostatin (mEnd) or human soluble FLK-1 (hsFLK-1) into a quadriceps muscle of C57BL/6 mice. The highest plasma levels of therapeutic protein were observed in AAV8-injected mice (8 > 7 > 1 > 2). Sustained expression of mEnd was detected for 6 months, whereas concentrations of hsFLK-1 declined to the background level within 2 weeks caused by neutralizing anti-hsFLK-1 antibody. These data demonstrate that AAV8 (mEnd) serotype is the most efficient mediator for protein expression.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Transferência de Genes
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Dependovirus
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Receptor 2 de Fatores de Crescimento do Endotélio Vascular
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Endostatinas
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Músculo Quadríceps
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Vetores Genéticos
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
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Humans
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Male
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article