Deciphering enzyme function using peptide arrays.
Mol Biotechnol
; 49(3): 283-305, 2011 Nov.
Article
em En
| MEDLINE
| ID: mdl-21604200
ABSTRACT
Enzymes are key molecules in signal-transduction pathways. However, only a small fraction of more than 500 human kinases, 300 human proteases and 200 human phosphatases is characterised so far. Peptide microarray based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last years. This technology reduces set-up time for HTS assays and allows the identification of downstream targets. Moreover, peptide microarrays enable optimisation of enzyme substrates. Focus of this review is on assay principles for measuring activities of kinases, phosphatases or proteases and on substrate identification/optimisation for kinases. Additionally, several examples for reliable identification of substrates for lysine methyl-transferases, histone deacetylases and SUMO-transferases are given. Finally, use of high-density peptide microarrays for the simultaneous profiling of kinase activities in complex biological samples like cell lysates or lysates of complete organisms is described. All published examples of peptide arrays used for enzyme profiling are summarised comprehensively.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Análise Serial de Proteínas
/
Enzimas
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article