Gold-nanoparticle-based graphite furnace atomic absorption spectrometry amplification and magnetic separation method for sensitive detection of mercuric ions.
Biosens Bioelectron
; 26(11): 4605-9, 2011 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-21605967
We have developed a sensitive gold-nanoparticle-based (AuNP-based) graphite furnace atomic absorption spectrometry (GFAAS) amplification and magnetic separation method for the detection of mercuric ions (Hg²âº). The assay relies on (i) a sandwich-type structure containing two thymine-thymine (T-T) mismatches for selectively recognizing Hg²âº ions; (ii) magnetic beads for homogeneous separation; and (iii) AuNP-based GFAAS amplification detection. The limit of detection (LOD) of this assay is 0.45 nM (0.09 µg L⻹)--one order of magnitude lower than the United States Environmental Protection Agency (US EPA) limit for Hg²âº in drinking water. Furthermore, because a shorter hybridization step and a simpler AuNP-based GFAAS amplification detection were employed, a faster analytical run time allowing us to analyze a batch of 24 samples within 0.5 h. We demonstrated the feasibility of the developed approach for the determination of Hg²âº in urine and aqueous environmental samples.
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1
Base de dados:
MEDLINE
Assunto principal:
Espectrofotometria Atômica
/
Técnicas Biossensoriais
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Mercúrio
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article