A unique paradigm for a Turn-ON near-infrared cyanine-based probe: noninvasive intravital optical imaging of hydrogen peroxide.
J Am Chem Soc
; 133(28): 10960-5, 2011 Jul 20.
Article
em En
| MEDLINE
| ID: mdl-21631116
ABSTRACT
The development of highly sensitive fluorescent probes in combination with innovative optical techniques is a promising strategy for intravital noninvasive quantitative imaging. Cyanine fluorochromes belong to a superfamily of dyes that have attracted substantial attention in probe design for molecular imaging. We have developed a novel paradigm to introduce a Turn-ON mechanism in cyanine molecules, based on a distinctive change in their π-electrons system. Our new cyanine fluorochrome is synthesized through a simple two-step procedure and has an unprecedented high fluorescence quantum yield of 16% and large extinction coefficient of 52,000 M(-1)cm(-1). The synthetic strategy allows one to prepare probes for various analytes by introducing a specific triggering group on the probe molecule. The probe was equipped with a corresponding trigger and demonstrated efficient imaging of endogenous hydrogen peroxide, produced in an acute lipopolysaccharide-induced inflammation model in mice. This approach provides, for the first time, an available methodology to prepare modular molecular Turn-ON probes that can release an active cyanine fluorophore upon reaction with specific analyte.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Carbocianinas
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Fenômenos Ópticos
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Imagem Molecular
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Corantes Fluorescentes
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Peróxido de Hidrogênio
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Raios Infravermelhos
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article