Poly[N-(2-hydroxypropyl)methacrylamide]-based tissue-embedding medium compatible with MALDI mass spectrometry imaging experiments.
Anal Chem
; 83(13): 5458-62, 2011 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-21634803
ABSTRACT
Traditional tissue-sectioning techniques for histological samples utilize various embedding media to stabilize the tissue on a sectioning target and to provide a smooth cutting surface. Due to the ion suppression effect in MALDI ionization and number of background peaks in the low-mass region, these media are not suitable for mass spectrometry imaging (MSI) experiments. To overcome this, droplets of water are often used to mount the tissue on a sectioning target, but the ice block formed around the tissue does not provide a good support for sectioning of fragile samples. In this work, we propose a novel embedding media, compatible with MALDI ionization and MSI experiments, based on poly[N-(2-hydroxypropyl)methacrylamide] (pHPMA). Using a reversible addition-fragmentation chain transfer polymerization technique, well-defined pHPMA polymer with narrow mass distribution was prepared. Benefits of the resulted pHPMA-based embedding media were tested on different tissue samples.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polímeros
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Acrilamidas
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Inclusão do Tecido
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Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article