[Clinic research of the effect of bFGF on human degenerated nucleus pulposus cells].

ABSTRACT

AIM: To determine the effect of bFGF on extracellular matrix and gene expression on the human de-generated nucleus pulposus cells. METHODS: The degenerated intervertebral disc cells were divided into 5 groups. A group adding 100 pg/L bFGF, B group adding 200 pg/LbFGF, C group 500 pg/L bFGF, D group 1000 pg/L bF-GF, E groups control group, without interfering factors. The type II collagen and GAG mRNA expression were test, type II collagen content and glycosaminoglycan content of the supernatant were test. RESULTS: bFGF stimulated de-generated nucleus pulposus cells, collagen II, GAG mRNA, collagen type II and glycosaminoglycan expression of the extracellular matrix were increased significantly on the 7 days compared with the control group (P < 0. 05). type II collagen and GAG mRNA were significantly lower than the control group on 14 days, 21 days (P < 0.05). However,collagen type II and glycosaminoglycan expression of the extracellular matrix on 14 days, 21 days were still higher than normal (P <0.05). CONCLUSION: bFGF increase GAG and type II collagen mRNA expression, increased extracellular matrix type II collagen and GAG expression in short-term.