Development of a 3D pigmented skin model to evaluate RNAi-induced depigmentation.
Exp Dermatol
; 20(9): 773-5, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-21707757
ABSTRACT
Because current skin whitening agents often have insufficient efficacy and side effects, we aim to develop effective and safe therapeutics using RNA interference (RNAi). We established a pigmented human-reconstructed skin model as a first step in the development of novel siRNA-based depigmenting agents. Histological characterization revealed that our model had a similar morphology as normal human skin, expressed keratinocyte differentiation as well as basement membrane markers, and showed a high degree of pigmentation. The utility of the model to study RNAi-induced depigmentation was validated by incorporation of melanocytes transfected with siRNA against tyrosinase, a key enzyme in skin pigmentation. This resulted in a strong reduction in pigmentation and inhibition of melanin transfer proving that siRNA-mediated gene silencing in melanocytes worked successfully in our model. Therefore, this self-made 3D skin model will be a useful and easy tool to validate the whitening potential of candidate genes with a presumed function in melanin synthesis or transfer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pigmentação da Pele
/
Interferência de RNA
/
Modelos Biológicos
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article