[Effect of different intensities of electroacupuncture on expression of monocyte chemoattractant protein-1 and TNF-alpha in adipose tissue in obesity rats].
Zhen Ci Yan Jiu
; 36(2): 79-84, 2011 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-21717772
ABSTRACT
OBJECTIVE:
To observe the effect of different intensities of electroacupuncture (EA) on adipose tissue inflammatory cytokines in rats with simple obesity so as to investigate its mechanism underlying body weight reduction.METHODS:
Forty SD male rats were randomly divided into normal, model, strong EA and weak EA groups (n = 10/group). Obesity model was established by feeding the rats with high fat diet. EA (20 Hz, strong EA 5 V, weak EA 2. 5 V) was applied to "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) for 15 min, once everyday and for 14 days. Body weight and Lee's index (= body weight(1/3) x 10(3) / body length) were detected. The fasting blood glucose was detected by hexokinase method, serum triglyceride (TG) was detected by glycerol-phosphoric acid oxidase peroxydase (GPO-POD)method, total cholesterol (TC) was detected by cholesterol oxidase method, high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-O) were measured by using one-step method, respectively. The expression of monocyte chemoattractant protein-1 (MOP-1) mRNA and tumor necrosis factor (TNF)-alpha mRNA in the epididymis adipose tissue was detected by reverse transcription-polymerase chain reaction (RT-POR).RESULTS:
In comparison with the normal group, the body weight, Lee's index, blood lipid (TG, TC, LDL-C), fasting blood glucose levels, and expression of MOP-1 mRNA and TNF-alpha mRNA were significantly higher in the model group (P < 0.01), and HDL-C was significantly lower (P < 0.01). After EA,compared with the model group, the body weight, Lee's index, TG, TC, LDL-C, fasting blood glucose levels, and expression of MCP-1 mRNA and TNF-a mRNA in both strong and weak EA groups were significantly decreased (P < 0.01, P < 0.05), and HDL-C was significantly increased (P < 0.01, P < 0.05). The effects of strong EA group were obviously superior to those of weak EA group (P < 0.05, P < 0.01). No statistical significance was observed between the two EA groups in fasting blood glucose levels (P > 0.05).CONCLUSION:
EA of "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) has a beneficial weight-reduction effect on rats with simple obesity, and moreover, the effect of strong EA stimulation is evidently superior to weak EA stimulation.
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Base de dados:
MEDLINE
Assunto principal:
Eletroacupuntura
/
Tecido Adiposo
/
Fator de Necrose Tumoral alfa
/
Quimiocina CCL2
/
Obesidade
Tipo de estudo:
Clinical_trials
/
Evaluation_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2011
Tipo de documento:
Article