[Detection of enteroviruses in urban wastewater in Yopougon, Abidjan]. / Détection d'entérovirus dans les eaux usées urbaines à Yopougon, Abidjan.

ABSTRACT

AIM: The aim of this study was to determine prevalence of enteroviruses in raw municipal wastewater and specify serotypes circulating. METHODS: After virus concentration by WHO recommend method, aqueous phase of 68 samples was inoculate on BGM, RD, L20B and Hep2 cells lines. Typing after titration was performed by microplate neutralization test with specific horse immune serum against EV (kit RIVM/WHO). NPEV strains were confirmed by RT-PCR test in 5'NC genomic region. Antigenic and molecular tests of Polioviruses intratypic differentiation were performed to determine vaccine or wild origin of Poliovirus strains. RESULTS: Viral isolation obtained 43/68 (65.15 %) positives samples. Among these 35/43 (81. 39%) were EV and 8/43 (19.04 %) non EV strain. Among EVs, 34/35 (97.14 %) were NEPV and 2/35 (5.71 %) vaccinal Poliovirus strains type 2 (Sabin-like). NEPV typing gave 77 % untypeable strains by conventionally method. The average virus concentration estimated in the raw sewage is 4.28×10(5) PFU/L. CONCLUSION: Various contacts between people and these waters expose them to health risks.