Transversal inducing differentiation of human amniotic epithelial cells into hepatocyte-like cells.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
; 36(6): 525-31, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21743144
ABSTRACT
OBJECTIVE:
To evaluate the in vitro differentiation of human amniotic epithelial cells (hAECs ) into hepatocyte-like cells.METHODS:
Combined approach of dexamethasone, HGF, IGF and other cytokines were used to induce the differentiation of hAECs into hepatocyte-like cells. The induction lasted 2 weeks. During the induction, the expression of albumin ALB, CYP1A1, CYP1A2, IGFR, c-met and key functional genes related to liver cells as well as transcription factors HNF3, HNF4 and C/EBPa were monitored by RT-PCR. Time dependent changes of the surface marker colony ALB, AFP and CK18 were analyzed by cell flow cytometry.RESULTS:
After the 2 week induction, the expressions of liver hepatocyte-like cell functional genes such as albumin, CYP1A1, CYP1A2, c-met, and transcription factors such as HNF3, HNF4, C/EBPa and HNF1 were observed. Six days after the induction, hAECs mainly were stained AFP+, and the positive rate was (15.1 ± 2.1)%. While 10 days after the induction, part of the hAECs showed AFP+/ALB+ (6.5 ± 1.4)%; and on 14th day, hAECs only showed ALB+, and the rate was (13.9 ± 2.3)%. ALB+ cell increase indicated a gradual functional maturation from the hAECs to hepatocyte-like cells. Similaritly, the number of CK18+ cells in the whole population was also increased On 10th day, the rate was (16.1 ± 1.2)%; on 14th day, that was (21.3 ± 4.6)%, which proved the above hypothesis of the trandifferentiation. By extending the induction time, the expression of functional genes increased gradually, and a maturing process of hAECs was detected by cell surface markers.CONCLUSION:
The differentiation of hAECs induced in vitro has the characteristics of hepatocyte-like cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Hepatócitos
/
Células Epiteliais
/
Âmnio
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article