[Determination of evodiamine and rutacarpine in zhuyu hewei zhitong capsules by HPLC].

ABSTRACT

OBJECTIVE: To develop a HPLC method for the determination of evodiamine and rutacarpine in Zhuyu Hewei Zhitong capsules. METHOD: The separation was performed at 30 degrees C on an Eclipse XDB-C18 column (4.6 mm x 150 mm, 5 microm) eluted with the mobile phase consisted of acetonitrile-water(4555). The detection wavelength was 250 nm. RESULTS: The standard curve for evodiamine is linear in the range of 0.0510-0.560 microg, the average recovery was 97.5% (RSD 1.0%). The standard curve for rutacarpine is linear in the range of 0.0508-0.559 microg, the average recovery was 98.7% (RSD 1.5%). CONCLUSION: The method is specific, accurate and stable. It can be used for detemination of evodiamine and rutacarpine in Zhuyu Hewei Zhitong capsules.