Improved detection of respiratory viruses in pediatric outpatients with acute respiratory illness by real-time PCR using nasopharyngeal flocked swabs.
J Clin Microbiol
; 49(9): 3365-7, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-21775539
ABSTRACT
Detection of respiratory viruses by real-time multiplexed PCR (M-PCR) and of respiratory syncytial virus (RSV) by M-PCR and immunofluorescence (IF) was evaluated using specimens collected by nasopharyngeal flocked swabbing (NFS) and nasal washes (NW). In children with mild respiratory illness, NFS collection was superior to NW collection for detection of viruses by M-PCR (sensitivity, 89.6% versus 79.2%; P = 0.0043). NFS collection was noninferior to NW collection in the detection of RSV by IF.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sistema Respiratório
/
Infecções Respiratórias
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Manejo de Espécimes
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Vírus
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Viroses
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Técnicas de Diagnóstico Molecular
/
Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Child, preschool
/
Female
/
Humans
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Infant
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Male
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article