Calcium sulfate stimulates pulp stem cells towards osteoblasts differentiation.
Int J Immunopathol Pharmacol
; 24(2 Suppl): 51-7, 2011.
Article
em En
| MEDLINE
| ID: mdl-21781446
ABSTRACT
Calcium sulfate (CaS) is a highly biocompatible material and enhances bone formation in vivo. However, how CaS alters osteoblast activity to promote bone formation is poorly understood. To study how CaS can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were compared in normal osteoblasts and dental pulp stem cells, using real time Reverse Transcription-Polymerase Chain Reaction. Gene differentially expressed between the two cells type were the trascriptional factor RUNX2, osteopontin (SPP1), COL1A1 (collagen type 1α1) and alkaline phosphatase (ALPL). The obtained results demonstrated that CaS strongly influences the behavior of DPSCs in vitro enhancing proliferation, differentiation and deposition of matrix.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Sulfato de Cálcio
/
Regulação da Expressão Gênica
/
Polpa Dentária
Limite:
Adult
/
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article