[Effect of bortezomib on the drug sensitivity of imatinib resistant K562/G01 cells].
Zhonghua Xue Ye Xue Za Zhi
; 32(6): 392-5, 2011 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-21781497
ABSTRACT
OBJECTIVE:
To explore the effect of bortezomib (BOR) on the drug sensitivity of imatinib-resistant chronic myeloid leukemia cell line K562/G01 cell and its mechanism.METHODS:
MTT assay was used to detect the inhibition effect of cell growth, flow cytometry to cell cycle, and real time-PCR to the expression of COX-2 and mdr1 mRNA.RESULTS:
Combination of 10 and 20 nmol/L BOR with imatinib could significantly enhance the sensitivity of K562/G01 to imatinib, the reverse factor was 1.83 and 2.72-fold respectively. Cell cycle arrested at G(2)/M phase could be observed by flow cytometry on BOR treatment. The over-expression of COX-2 and mdr1 could be down-regulated by BOR.CONCLUSIONS:
BOR can enhance the imatinib sensitivity of imatinib resistant K562/G01 cell. The mechanism may be related to cell cycle phase arrested at G2/M and down-regulation of COX-2 and mdr1 expression.
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Base de dados:
MEDLINE
Assunto principal:
Piperazinas
/
Pirazinas
/
Pirimidinas
/
Ácidos Borônicos
/
Resistencia a Medicamentos Antineoplásicos
/
Antineoplásicos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2011
Tipo de documento:
Article