The immunological potency and therapeutic potential of a prototype dual vaccine against influenza and Alzheimer's disease.
J Transl Med
; 9: 127, 2011 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-21806809
ABSTRACT
BACKGROUND:
Numerous pre-clinical studies and clinical trials demonstrated that induction of antibodies to the ß-amyloid peptide of 42 residues (Aß42) elicits therapeutic effects in Alzheimer's disease (AD). However, an active vaccination strategy based on full length Aß42 is currently hampered by elicitation of T cell pathological autoreactivity. We attempt to improve vaccine efficacy by creating a novel chimeric flu vaccine expressing the small immunodominant B cell epitope of Aß42. We hypothesized that in elderly people with pre-existing memory Th cells specific to influenza this dual vaccine will simultaneously boost anti-influenza immunity and induce production of therapeutically active anti-Aß antibodies.METHODS:
Plasmid-based reverse genetics system was used for the rescue of recombinant influenza virus containing immunodominant B cell epitopes of Aß42 (Aß1-7/10).RESULTS:
Two chimeric flu viruses expressing either 7 or 10 aa of Aß42 (flu-Aß1-7 or flu-Aß1-10) were generated and tested in mice as conventional inactivated vaccines. We demonstrated that this dual vaccine induced therapeutically potent anti-Aß antibodies and anti-influenza antibodies in mice.CONCLUSION:
We suggest that this strategy might be beneficial for treatment of AD patients as well as for prevention of development of AD pathology in pre-symptomatic individuals while concurrently boosting immunity against influenza.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vacinas contra Influenza
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Influenza Humana
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Doença de Alzheimer
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article