Regulation of the calpain and ubiquitin-proteasome systems in a canine model of muscular dystrophy.
Muscle Nerve
; 44(4): 553-62, 2011 Oct.
Article
em En
| MEDLINE
| ID: mdl-21826685
ABSTRACT
INTRODUCTION:
Previous studies have tested the hypothesis that calpain and/or proteasome inhibition is beneficial in Duchenne muscular dystrophy, based largely on evidence that calpain and proteasome activities are enhanced in the mdx mouse.METHODS:
mRNA expression of ubiquitin-proteasome and calpain system components were determined using real-time polymerase chain reaction in skeletal muscle and heart in the golden retriever muscular dystrophy model. Similarly, calpain 1 and 2 and proteasome activities were determined using fluorometric activity assays.RESULTS:
We found that less than half of the muscles tested had increases in proteasome activity, and only half had increased calpain activity. In addition, transcriptional regulation of the ubiquitin-proteasome system was most pronounced in the heart, where numerous components were significantly decreased.CONCLUSION:
This study illustrates the diversity of expression and activities of the ubiquitin-proteasome and calpain systems, which may lead to unexpected consequences in response to pharmacological inhibition.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Calpaína
/
Músculo Esquelético
/
Ubiquitina
/
Complexo de Endopeptidases do Proteassoma
/
Distrofia Muscular Animal
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article