Proteolipidic composition of exosomes changes during reticulocyte maturation.
J Biol Chem
; 286(39): 34426-39, 2011 Sep 30.
Article
em En
| MEDLINE
| ID: mdl-21828046
During the orchestrated process leading to mature erythrocytes, reticulocytes must synthesize large amounts of hemoglobin, while eliminating numerous cellular components. Exosomes are small secreted vesicles that play an important role in this process of specific elimination. To understand the mechanisms of proteolipidic sorting leading to their biogenesis, we have explored changes in the composition of exosomes released by reticulocytes during their differentiation, in parallel to their physical properties. By combining proteomic and lipidomic approaches, we found dramatic alterations in the composition of the exosomes retrieved over the course of a 7-day in vitro differentiation protocol. Our data support a previously proposed model, whereby in reticulocytes the biogenesis of exosomes involves several distinct mechanisms for the preferential recruitment of particular proteins and lipids and suggest that the respective prominence of those pathways changes over the course of the differentiation process.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Endossomos
/
Reticulócitos
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Diferenciação Celular
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Lipídeos de Membrana
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Proteínas de Membrana
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article