Goodpasture antigen-binding protein (GPBP) directs myofibril formation: identification of intracellular downstream effector 130-kDa GPBP-interacting protein (GIP130).
J Biol Chem
; 286(40): 35030-43, 2011 Oct 07.
Article
em En
| MEDLINE
| ID: mdl-21832087
ABSTRACT
Goodpasture antigen-binding protein-1 (GPBP-1) is an exportable non-conventional Ser/Thr kinase that regulates glomerular basement membrane collagen organization. Here we provide evidence that GPBP-1 accumulates in the cytoplasm of differentiating mouse myoblasts prior to myosin synthesis. Myoblasts deficient in GPBP-1 display defective myofibril formation, whereas myofibrils assemble with enhanced efficiency in those overexpressing GPBP-1. We also show that GPBP-1 targets the previously unidentified GIP130 (GPBP-interacting protein of 130 kDa), which binds to myosin and promotes its myofibrillar assembly. This report reveals that GPBP-1 directs myofibril formation, an observation that expands its reported role in supramolecular organization of structural proteins to the intracellular compartment.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Sanguíneas
/
Proteínas de Transporte
/
Regulação da Expressão Gênica
/
Proteínas Serina-Treonina Quinases
/
Proteínas de Ligação a DNA
/
Miofibrilas
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article