Radiosensitization of head and neck cancer cells by the phytochemical agent sulforaphane.
Strahlenther Onkol
; 187(9): 575-80, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-21858418
ABSTRACT
BACKGROUND AND PURPOSE:
Sulforaphane is a naturally occurring compound found in broccoli and other cruciferous vegetables. Recently it gained attention because of its antiproliferative properties in many cancer cell lines. The aim of this study was to investigate whether sulforaphane could act as a radiosensitizer in head and neck squamous cell carcinoma cell lines. MATERIALS ANDMETHODS:
Four head and neck squamous cell carcinoma cell lines (i.e., (HNSCC) SCC9, SCC25, CAL27, and FADU) were treated with sulforaphane and subsequently irradiated. Then proliferation and clonogenic assays were performed. Apoptosis was detected by flow cytometry. Possible regulation of Akt and Mcl-1 was investigated by western blotting.RESULTS:
Sulforaphane and radiation in combination leads to stronger inhibition of cell proliferation and of clonogenic survival than each treatment method alone. Western blot analysis of Akt and Mcl-1 showed no changed expression.CONCLUSION:
Sulforaphane is a promising agent in the treatment of head and neck cancer due to its antiproliferative and radio-sensitizing properties. A combination of sulforaphane and radiation decreases clonogenic survival. Apoptosis is not regulated through Akt or the Mcl-1 protein.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Radiossensibilizantes
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Tiocianatos
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Carcinoma de Células Escamosas
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Ensaio Tumoral de Célula-Tronco
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Divisão Celular
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Anticarcinógenos
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Apoptose
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Neoplasias de Cabeça e Pescoço
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Fitoterapia
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article